| Date | Home | Result | Away | |
| 13-02-21 | Gloucester City | 1 - 1 |
Chorley | |
| 09-01-21 | Gloucester City | 3 - 2 |
Kidderminster Harriers | |
| 02-01-21 | Leamington | 1 - 1 |
Gloucester City | |
| 28-12-20 | Brackley Town | 3 - 2 |
Gloucester City | |
| 26-12-20 | Gloucester City | 1 - 0 |
Leamington | |
| 12-12-20 | Curzon Ashton | 0 - 1 |
Gloucester City | |
| 01-12-20 | Gloucester City | 0 - 0 |
Boston United | |
| 28-11-20 | Gloucester City | 1 - 2 |
Farsley Celtic | |
| 24-11-20 | Gateshead | 1 - 0 |
Gloucester City | |
| 21-11-20 | Guiseley | 1 - 2 |
Gloucester City | |
| 17-11-20 | Hereford FC | 0 - 1 |
Gloucester City | |
| 14-11-20 | Gloucester City | 4 - 4 |
Bradford Park Avenue | |
| 10-11-20 | Gloucester City | 4 - 1 |
AFC Telford United | |
| 07-11-20 | Spennymoor Town | 2 - 2 |
Gloucester City | |
| 31-10-20 | Gloucester City | 2 - 1 |
Chester | |
| 17-10-20 | Gloucester City | 6 - 1 |
Blyth Spartans | |
| 10-10-20 | Chorley | 1 - 2 |
Gloucester City | |
| 06-10-20 | Gloucester City | 3 - 1 |
Kettering Town |